Part:BBa_K3956005:Experience

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Applications of BBa_K3956005

This protein domain was inserted in between a synthetic promoter and enzyme cut site to express bacterial collagen in yeast.

Although we were able to verify that the construct had been correctly inserted into appropriate selective plates by colony PCR verification, the protein expression was not able to be verified within the time constraints of the competition. We want to emphasize that this sequence may still be successful, but its efficiency was unable to be demonstrated within the time span that CCA_San_Diego had in the lab. For future teams, we advise testing construct designs with successful expression with and without the sequence to compare expression levels.

User Reviews

UNIQfc5a3d9e35352448-partinfo-00000000-QINU UNIQfc5a3d9e35352448-partinfo-00000001-QINU